Goat Secondary Antibodies

Secondary antibodies where goat is the host species. Secondary antibodies must be raised against the host species of the primary antibody. Includes different antigens, classifications, conjugations, and isotypes.

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4,561

Goat Anti-Rat IgG (H&L) Antibody (Alkaline Phosphatase), ProSci

Secondary Antibody

Pricing & Availability
Specifications

Content And Storage	Alkaline Phosphatase Conjugated Affinity Purified anti-Rat IgG can be stored vial at 4 °C before opening. DO NOT FREEZE. This product is stable at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Target Species	Rat
Host Species	Goat
Conjugate	Alkaline Phosphatase (AP)
Applications	ELISA,Western Blot,Immunohistochemistry
Form	Liquid
Isotype	IgG
Antigen	Goat Anti-Rat IgG (H&L)
Regulatory Status	RUO
Purification Method	Alkaline Phosphatase Conjugated Affinity Purified anti-Rat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.
Dilution	Alkaline Phosphatase Conjugated Affinity Purified anti-Rat IgG is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:4,000 - 1:20,000, Western Blot 1:1,000 - 1:5,000, Immunohistochemistry 1:200 - 1:1,000 . (Optimal dilution should be determined by user).
Product Type	Antibody
Formulation	0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.01% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA)
Classification	Polyclonal
Immunogen	Rat IgG - whole molecule
Primary or Secondary	Secondary
Test Specificity	Minimum Cross reactivity to Human Serum Proteins

4,562

Goat Anti-Rabbit IgG Antibody (H&L) Antibody (HRP), ProSci

Secondary Antibody

Pricing & Availability
Specifications

Content And Storage	Store 2 - 8 °C as an undiluted liquid for several weeks. After dilution, do not use for more than one day. -20 °C for up to one year.
Target Species	Rabbit
Host Species	Goat
Conjugate	HRP
Applications	ELISA,Western Blot,Immunohistochemistry,Immunocytochemistry
Form	Liquid
Isotype	IgG
Antigen	Goat Anti-Rabbit IgG
Regulatory Status	RUO
Dilution	1:250-1:3,000 for IHC/ICC, , 1:2,000-1:5,000 for ELISA, Western Blot, color substrates., , 1:5,000-1:10,000 for Western Blot, chemiluminescence.
Product Type	Antibody
Formulation	Buffer containing 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.6 and 25% glycerol.
Classification	Polyclonal
Immunogen	Purified rabbit IgG.
Primary or Secondary	Secondary
Test Specificity	This antibody reacts with both heavy and light chains of rabbit IgG, slight cross-reactivity with IgG of other mammalian species.